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Exhibit (32.1)

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Section 1350 Certifications

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Pursuant to 18 U.S.C. Section 1350, each of the undersigned officers of Ecolab Inc. does hereby certify that:

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(a)the Quarterly Report on Form 10-Q of Ecolab Inc. for the quarter ended March 31, 2026 (the “Report”) fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934; and

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(b)information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of Ecolab Inc.

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Dated: May 7, 2026

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/s/ Christophe Beck

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Christophe Beck

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Chief Executive Officer

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Dated: May 7, 2026

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/s/ Scott D. Kirkland

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Scott D. Kirkland

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Chief Financial Officer

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