Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We have issued our reports dated September 11, 2025, with respect to the consolidated financial statements and internal control over financial reporting included in the Annual Report of LSI Industries Inc. on Form 10-K for the year ended June 30, 2025. We consent to the incorporation by reference of said reports in the Registration Statements of LSI Industries Inc. on Form S-3 (File No. 333-267377) and on Forms S-8 (File No. 333-282098, File No. 333-282097, File No. 333-269634, File No. 333-260767, File No. 333-259452, File No. 333-249861, File No. 333-248747, File No. 333-234556, File No. 333-233661, File No. 333-201890, and File No. 333-183747).

/s/ GRANT THORNTON LLP

Chicago, Illinois

September 11, 2025