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Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statements on Form S-3 (Nos. 333-274029, 333-274030, 333-274028, 333-281814, 333-267462, 333-283684, 333-284943, 333-285007, 333-288505, 333-288504 and 333-290153) of Pruco Life Insurance Company of our report dated March 6, 2026 relating to the consolidated financial statements and financial statement schedules, which appears in this Form 10-K.

/s/ PricewaterhouseCoopers LLP

New York, New York

March 6, 2026