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Exhibit 8.1

The principal subsidiaries of NatWest Group plc are:

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Nature of business

Country of incorporation and
principal area of operation

Group interest

National Westminster Bank Plc (1,3)

Banking

Great Britain

100%

The Royal Bank of Scotland plc (3)

Banking

Great Britain

100%

Coutts & Company (2, 3)

Banking

Great Britain

100%

Ulster Bank Ireland Designated Activity Company (3)

Banking

Republic of Ireland

100%

NatWest Markets Plc

Banking

Great Britain

100%

NatWest Markets N.V. (4)

Banking

Netherlands

100%

The Royal Bank of Scotland International Limited (5)

Financial Institution

Jersey

100%

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(1)The company does not hold any of the preference shares in issue.

(2)Coutts & Company is incorporated with unlimited liability.

(3)Owned via NatWest Holdings Limited.

(4)Owned via NatWest Markets Plc.

(5)Owned via The Royal Bank of Scotland International (Holdings) Limited.

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The above information is provided only in relation to subsidiary companies which would be deemed to be a “significant subsidiary” in accordance with rule 1-02(w) of Regulation S-X as at 31 December 2022.

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