<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0000855683</cik>
        <entityName>MILESTONE SCIENTIFIC INC.</entityName>
        <issuerAddress>
            <street1>425 EAGLE ROCK AVENUE</street1>
            <street2>SUITE 403</street2>
            <city>ROSELAND</city>
            <stateOrCountry>NJ</stateOrCountry>
            <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
            <zipCode>07068</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>973-535-2717</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <previousName>U S OPPORTUNITY SEARCH, INC</previousName>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <previousName>MILESTONE SCIENTIFIC INC/NJ</previousName>
            <previousName>U S OPPORTUNITY SEARCH INC</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Eric</firstName>
                <lastName>Hines</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>425 EAGLE ROCK AVENUE</street1>
                <street2>SUITE 403</street2>
                <city>ROSELAND</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07068</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Executive Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Keisha</firstName>
                <lastName>Harcum</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>425 EAGLE ROCK AVENUE</street1>
                <street2>SUITE 403</street2>
                <city>ROSELAND</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07068</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Vice President of Finance</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Benedetta</firstName>
                <middleName>I.</middleName>
                <lastName>Casamento</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>425 EAGLE ROCK AVENUE</street1>
                <street2>SUITE 403</street2>
                <city>ROSELAND</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07068</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chairman of the Board</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Neal</firstName>
                <lastName>Goldman</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>425 EAGLE ROCK AVENUE</street1>
                <street2>SUITE 403</street2>
                <city>ROSELAND</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07068</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Didier</firstName>
                <lastName>Demesmin</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>425 EAGLE ROCK AVENUE</street1>
                <street2>SUITE 403</street2>
                <city>ROSELAND</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07068</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Shanth</firstName>
                <lastName>Thiyagalingam</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>425 EAGLE ROCK AVENUE</street1>
                <street2>SUITE 403</street2>
                <city>ROSELAND</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07068</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Dawood</firstName>
                <lastName>Sayed</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>425 EAGLE ROCK AVENUE</street1>
                <street2>SUITE 403</street2>
                <city>ROSELAND</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07068</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other Health Care</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2026-04-20</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>25000</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>4837500</totalOfferingAmount>
            <totalAmountSold>2150000</totalAmountSold>
            <totalRemaining>2687500</totalRemaining>
            <clarificationOfResponse>Total Offering Amount includes $2,687,500 of potential proceeds payable upon the exercise of warrants issued in the offering. Total Amount Sold includes $1,799,000 in cash and $351,000 from conversion of a portion of certain convertible bridge notes.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>21</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>MILESTONE SCIENTIFIC INC.</issuerName>
                <signatureName>/s/ Eric Hines</signatureName>
                <nameOfSigner>Eric Hines</nameOfSigner>
                <signatureTitle>Chief Executive Officer</signatureTitle>
                <signatureDate>2026-04-22</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>