Exhibit 23.01

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CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statements on Form S-8 (Nos. 333-252725, 333-202005, 333-216312, 333-221826, and 333-123242) of Azenta, Inc. of our report dated December 4, 2025 relating to the financial statements and the effectiveness of internal control over financial reporting, which appears in this Form 10-K.

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/s/ PricewaterhouseCoopers LLP
Boston, Massachusetts
December 4, 2025