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SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

SCHEDULE 13G

UNDER THE SECURITIES EXCHANGE ACT OF 1934

(Name of Issuer)

(Title of Class of Securities)

(CUSIP Number)

(Date of Event Which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

Rule 13d-1(b)

Rule 13d-1(c)

Rule 13d-1(d)

SCHEDULE 13D/A 0001654954-21-004737 0001859309 XXXXXXXX LIVE 10 Common Stock, par value $0.01 per share 03/10/2025 false 0001014763 00902F303 Ainos, Inc. 8880 RIO SAN DIEGO DRIVE SUITE 800 SAN DIEGO CA 92108 Chun-Hsien Tsai 886-37-581999 14F., No. 61, Sec. 4, New Taipei Bouleva Xinzhuang District New Taipei City 242 F5 F5 0001859309 N Ainos, Inc. a OO N E9 13853433.00 0.00 2456319.00 11397114.00 2456319.00 N 12.10 CO 1, Includes (i) 2,456,319 shares of common stock, $0.01 par value, of Ainos, Inc., a Texas corporation (the "Issuer"), owned directly by Ainos Inc., a Cayman Islands company ("Ainos KY"), (ii) 2,015,594 shares pursuant to a Voting Agreement dated January 26, 2024 (the "2024 Voting Agreement"), by and among the Issuer, Ainos Inc., and Chun-Hsien Tsai, Ting Chuan Lee, Chun-Jung Tsai, and Chung-Yi Tsai (the "Tsai Group"); (iii) 456,356 shares of common stock pursuant to a Voting Agreement dated March 7, 2024 (the "2024 Voting Agreement II") with Chih-Heng Lu; (iv) 3,148,788 shares pursuant to a Voting Agreement dated May 3, 2024 between Ainos KY and ASE Test, Inc. ("ASE Test" and the "ASE Voting Agreement") (with the 3,148,788 ASE Test shares consisting of the following (a) 29,411 shares owned by ASE Test, (b) 499,377 shares issuable to ASE Test upon conversion of outstanding convertible notes of the Issuer (c) 2,120,000 shares issuable to ASE Test upon conversion of a convertible note of the Issuer issuable within 60 days), and (d) 500,000 shares issuable to ASE Test upon exercise of warrants to purchase 500,000 shares of the Issuer with ASE Test, (v) 5,500,000 shares pursuant to a Voting Agreement dated August 15, 2024 between Ainos KY and Taiwan Carbon Nano Technology Corporation, and (vi) 276,376 shares pursuant to a Voting Agreement dated March 10, 2025 between Ainos KY and Hsin-Liang Lee (the "2025 Voting Agreement"). 2 Represents beneficial ownership of 2,456,319 shares of common stock of the Issuer, consisting of 2,456,319 shares owned directly by Ainos KY and excludes (i) 2,015,594 shares pursuant to the 2024 Voting Agreement; (ii) 456,356 shares of common stock pursuant to the 2024 Voting Agreement II; (iii) 3,148,788 shares pursuant to the ASE Voting Agreement as detailed in Note 1, (iv) 5,500,000 shares pursuant to a Voting Agreement dated August 15, 2024 between Ainos KY and Taiwan Carbon Nano Technology Corporation, and (v) 276,376 shares pursuant to the 2025 Voting Agreement. 3 Based on the sum of (i) 15,433,257 shares of Common Stock outstanding as of March 7, 2025 as set forth in the Annual Report on Form 10-K of the Issuer filed with the SEC on March 7, 2025, (ii) 1,752,500 shares of common stock granted and vested on March 10, 2025 as special stock awards, which were approved by the shareholders of Ainos, Inc. on September 27, 2024, and (iii) 3,119,377 total shares issuable to ASE Test upon conversion of outstanding convertible note and exercisable warrant of the Issuer. This Amendment No. 10 to Schedule 13D ("Amendment No. 10") amends and supplements the Schedule 13D dated April 28, 2021 (the "Original Statement"), as amended and restated by Amendment No. 1 dated December 13, 2023, as amended by Amendment No. 2 dated March 8, 2022, as amended by Amendment No. 3 dated September 2, 2022, as amended by Amendment No. 4 dated August 15, 2023, as amended by Amendment No. 5 dated January 29, 2024, as amended by Amendment No. 6 dated March 11, 2024, as amended by Amendment No. 7 dated May 6, 2024, as amended by Amendment No.8 dated August 20, 2024, as amended by Amendment No.9 dated November 26, 2024 (the Original Statement, as so amended shall be known as the "Statement"), with respect to the (i) the common stock, par value $0.01 per share (the "Common Stock"), of the Issuer. Unless otherwise indicated, all capitalized terms used herein shall have the same meaning as set forth in the Statement. Except as set forth below, there are no changes to the information set forth in the Statement. Items 2, 3, 4, 5 and 6 of the Statement are hereby amended as follows: Common Stock, par value $0.01 per share Ainos, Inc. 8880 RIO SAN DIEGO DRIVE SUITE 800 SAN DIEGO CA 92108 (a) This Schedule is being filed by Ainos Inc., a Cayman Islands corporation ("Ainos KY"). The principal business and office address of Ainos KY is P. O. Box 31119 Grand Pavilion, Hibiscus Way, 802 West Bay Road, Grand Cayman, KY1 - 1205 Cayman Islands. (b) Ainos KY is controlled by Taiwan Carbon Nano Technology Corporation, a Taiwanese corporation ("TCNT" and together with the Issuer, the "Parties"). The principal business and office address of TCNT is 10F-2, No. 66, Shengyi 5th Rd., Zhubei City, Hsinchu County 30261, Taiwan (R.O.C.). (c) The principal business of Ainos KY is a holding company of the Issuer's securities. The principal business of TCNT is the development of advanced materials for industrial and medical device applications. See Schedules A and B for information about the directors and executive officers of the Parties. (d-e) During the last five years, the reporting person and respective executive officers and directors, as applicable, have not (i) been convicted in a criminal proceeding (excluding traffic violations or similar misdemeanors) or (ii) been a party to a civil proceeding of a judicial or administrative body of competent jurisdiction and as a result of such proceeding was or is subject to a judgment, decree or final order enjoining future violations of, or prohibiting or mandating activities subject to, federal or state securities laws or finding any violation with respect to such laws. Since the filing of Amendment No. 9, (1) on March 10, 2025, the compensation committee of the issuer's board of directors granted 200,000, 200,000, 200,000, 150,000, 302,500, and 250,000 shares of common stock to Chun-Hsien Tsai, Ting Chuan Lee, Chun-Jung Tsai, Chung-Yi Tsai, Chih-Heng Lu, and Hsin-Liang Lee, respectively, as special stock awards, which fully vested on the same day. The special stock awards were approved the shareholders of Ainos, Inc. on September 27, 2024; (2) 1,468 restricted stock units ("RSUs"), owned by Chung-Yi Tsai, vested on January 31, 2025, in connection with RSUs granted to Chung-Yi Tsai under the Ainos, Inc. 2021 Stock Incentive Plan, as amended by the 2023 Stock Incentive Plan; and (3) on March 10, 2025, the Company entered in to an amendment to the convertible note, dated March 13, 2023, which increased the number of shares issuable to ASE Test upon conversion of the convertible note by 836,711 shares, as previously reported by the Issuer. Under the 2024 Voting Agreement, the 2024 Voting Agreement II, ASE Voting Agreement, and the 2025 Voting Agreement, Ainos KY has the sole discretion to determine the vote of all the parties' voting stock of the Issuer. The information requested by this Item 3 is incorporated herein by reference to Item 5 hereof. Ainos KY acquired the Common stock in the Issuer for investment purposes. Except as disclosed herein, each of the Parties has no plans which relate to or would result in an event described in subparagraphs (a) through (j) of Item 4 of Schedule 13D. However, each of the Parties may, from time to time, engage in discussions, whether initiated by the Parties or another party, concerning proposals for transactions or other arrangements that may relate to or, if consummated, result in an event described in Item 4 of Schedule 13D. Each of the Parties may review and evaluate their respective investments in the Issuer at any time, whether in light of the discussions described in the immediately preceding sentence or otherwise, which may give rise to plans or proposals that, if consummated, would result in one or more of the events described in Item 4 of Schedule 13D. Any such discussion or actions may consider various factors, including, without limitation, the Issuer's business prospects and other developments concerning the Issuer, alternative investment opportunities, general economic conditions, financial and stock market conditions and any other facts and circumstances that may become known to the Parties regarding or related to the matters described in this Statement. (a) - (b) The information requested by these paragraphs are incorporated herein by reference to the cover pages to this Amendment No. 10. (c) Since the most recent filing of Amendment No. 9 to Schedule 13D on November 27, 2024, the following transactions have occurred: (1) The Tsai Group acquired 750,000 shares of common stock on March 10, 2025, upon the grant and vesting such shares as special stock awards, with the Issuer's stock price on the vesting date being $0.46 per share; and (ii) 1,468 shares of common stock on January 31, 2025, in connection with the vesting of the RSUs granted to Chung-Yi Tsai under the 2021 Stock Incentive Plan, as amended by the 2023 Stock Incentive Plan, with the Issuer's stock price on the vesting date being $0.68 per share. (2) Chih-Heng Lu acquired 302,500 shares of common stock on March 10, 2025, upon the grant and vesting of such shares of common stock as special stock awards, with the Issuer's stock price on the vesting date being $0.46 per share. (3) ASE Test acquired 836,711 shares of common stock issuable upon exercise of the convertible note, dated March 13, 2023 and as amended on March 10, 2025. (4) Hsin-Liang Lee acquired 250,000 shares of common stock on March 10, 2025, upon the grant and vesting the such shares of common stock as special stock awards, and entered into the 2025 Voting Agreement with Ainos KY on the same day. The number of the total shares owned by Hsin-Liang Lee was 276,376 shares on March 10, 2025. (d) No other person is known to the Parties to have the right to receive or the power to direct the receipt of dividends or the proceeds from the sale of the Shares (other than their respective records owner). (e) Not applicable. There are no contracts, arrangements, understandings or relationships (legal or otherwise) among Ainos KY or the Parties and any other person with respect to any securities of the Issuer, including, but not limited to transfer or voting of any of the securities, finder's fees, joint ventures, loan or option arrangements, puts or calls, guarantees of profits, division of profits or loss, or the giving or withholding of proxies. Voting Agreement, dated March 10, 2025, between Ainos, Inc. and Hsin-Liang Lee. Ainos, Inc. /s/ Chun-Hsien Tsai Chun-Hsien Tsai Director and CEO 03/12/2025