United States securities and exchange commission logo January 25, 2024 Jose Humberto Acosta Martin Chief Financial Officer Bancolombia S.A. Carrera 48 # 26-85, Avenida Los Industriales Medellin, Colombia Re: Bancolombia S.A. Form 20-F for the Fiscal Year Ended December 31, 2022 Filed April 3, 2023 File No. 001-32535 Dear Jose Humberto Acosta Martin: We have completed our review of your filing. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Sincerely, Division of Corporation Finance Office of Finance