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Exhibit 8.1

LIST OF SUBSIDIARIES (EXHIBIT)

ENTITY

JURISDICTION OF INCORPORATION

BUSINESS

PROPORTION OF OWNERSHIP INTEREST AND VOTING POWER HELD BY THE BANK 2021

PROPORTION OF OWNERSHIP INTEREST AND VOTING POWER HELD BY THE BANK 2020

PROPORTION OF OWNERSHIP INTEREST AND VOTING POWER HELD BY THE BANK 2019

Fiduciaria Bancolombia S.A. Sociedad Fiduciaria

Colombia

Trust

98.81%

98.81%

98.81%

Banca de Inversión Bancolombia S.A. Corporación Financiera

Colombia

Investment banking

100.00%

100.00%

100.00%

Valores Bancolombia S.A. Comisionista de Bolsa

Colombia

Securities brokerage

100.00%

100.00%

100.00%

VLIPCO S.A.S.(1)

Colombia

Technology services provider

94.77%

-

-

Renting Colombia S.A.S.

Colombia

Operating leasing

100.00%

100.00%

100.00%

Transportempo S.A.S.

Colombia

Transportation

100.00%

100.00%

100.00%

Inversiones CFNS S.A.S.

Colombia

Investments

99.94%

99.94%

99.94%

Pasarela Colombia S.A.S.

Colombia

Payment solutions

100.00%

100.00%

100.00%

Fondo de Capital Privado Fondo Inmobiliario Colombia(2)

Colombia

Real estate investment fund

49.96%

49.96%

49.96%

P.A. Inmuebles CEM(2)

Colombia

Mercantil trust

49.96%

49.96%

-

P.A. Calle 92 FIC-11(2)

Colombia

Mercantil trust

32.47%

32.47%

-

P.A. FIC Edificio Corfinsura(2)

Colombia

Mercantil trust

49.96%

49.96%

-

P.A. FIC-A5(2)

Colombia

Mercantil trust

49.96%

49.96%

-

P.A. FIC Inmuebles(2)

Colombia

Mercantil trust

49.96%

49.96%

-

P.A. FIC Clínica de Prado(2)

Colombia

Mercantil trust

38.49%

38.49%

-

P. A. FIC A6(2)

Colombia

Mercantil trust

49.96%

49.96%

-

P.A. Central Point(2)

Colombia

Mercantil trust

37.47%

37.47%

-

Fideicomiso Irrevocable de Garantía, Fuente de Pago y Administración Inmobiliaria Polaris(2)

Colombia

Mercantil trust

49.96%

49.96%

-

P.A. Fideicomiso Twins Bay(2)

Colombia

Mercantil trust

49.96%

49.96%

-

Fideicomiso Lote Av San Martín(2)

Colombia

Mercantil trust

49.96%

49.96%

-

P.A. Fideicomiso Lote 30(2)

Colombia

Mercantil trust

49.96%

49.96%

-

Fideicomiso Fondo Inmobiliario Bancolombia(3)

Colombia

Mercantil trust

17.54%

-

-

Valores Simesa S.A.(4)

Colombia

Investments

66.82%

67.11%

67.11%

Fideicomiso Lote Abelardo Castro(5)

Colombia

Mercantil trust

-

66.77%

66.77%

Fideicomiso Lote Distrito Vera B1B2(4)

Colombia

Mercantil trust

66.49%

66.77%

66.77%

Fideicomiso Lote Distrito Vera B3B4(4)

Colombia

Mercantil trust

66.49%

66.77%

66.77%

Bancolombia Panamá S.A.

Panama

Banking

100.00%

100.00%

100.00%

Sistemas de Inversiones y Negocios S.A. Sinesa

Panama

Investments

100.00%

100.00%

100.00%

Banagrícola S.A.

Panama

Investments

99.17%

99.17%

99.17%

Banistmo S.A.

Panama

Banking

100.00%

100.00%

100.00%

Banistmo Investment Corporation S.A.

Panama

Trust

100.00%

100.00%

100.00%

Financomer S.A.(6)

Panama

Financial services

0.00%

100.00%

100.00%

Leasing Banistmo S.A.

Panama

Leasing

100.00%

100.00%

100.00%

Valores Banistmo S.A.

Panama

Securities brokerage

100.00%

100.00%

100.00%

Banistmo Panamá Fondo de Inversión S.A.

Panama

Holding

100.00%

100.00%

100.00%

Suvalor Renta Fija Internacional Corto Plazo S.A.

Panama

Collective investment fund

100.00%

100.00%

100.00%


ENTITY

JURISDICTION OF INCORPORATION

BUSINESS

PROPORTION OF OWNERSHIP INTEREST AND VOTING POWER HELD BY THE BANK 2021

PROPORTION OF OWNERSHIP INTEREST AND VOTING POWER HELD BY THE BANK 2020

PROPORTION OF OWNERSHIP INTEREST AND VOTING POWER HELD BY THE BANK 2019

Suvalor Colombia(7)

Panama

Collective investment fund

100.00%

100.00%

100.00%

Banistmo Capital Markets Group Inc.(7)

Panama

Purchase and sale of securities

100.00%

100.00%

100.00%

Anavi Investment Corporation S.A.(7)

Panama

Real estate

100.00%

100.00%

100.00%

Desarrollo de Oriente S.A.(7)

Panama

Real estate

100.00%

100.00%

100.00%

Steens Enterprises S.A.(7)

Panama

Portfolio holder

100.00%

100.00%

100.00%

Ordway Holdings S.A.(7)

Panama

Real estate broker

100.00%

100.00%

100.00%

Grupo Agromercantil Holding S.A.(8)

Panama

Holding

100.00%

100.00%

60.00%

Banco Agromercantil de Guatemala S.A.(8)

Guatemala

Banking

99.66%

99.59%

59.72%

Seguros Agromercantil de Guatemala S.A.(8)

Guatemala

Insurance agency

79.92%

79.90%

47.93%

Financiera Agromercantil S.A.(8)

Guatemala

Financial services

100.00%

100.00%

60.00%

Agrovalores S.A.(8)

Guatemala

Securities brokerage

100.00%

100.00%

60.00%

Arrendadora Agromercantil S.A.(8)

Guatemala

Operating Leasing

100.00%

100.00%

60.00%

Agencia de Seguros y Fianzas Agromercantil S.A.(8)(9)

Guatemala

Insurance company

100.00%

100.00%

60.00%

Asistencia y Ajustes S.A.(8)

Guatemala

Services

100.00%

100.00%

60.00%

Serproba S.A.(8)

Guatemala

Maintenance and remodelling services

100.00%

100.00%

60.00%

Servicios de Formalización S.A.(8)

Guatemala

Loans formalization

100.00%

100.00%

60.00%

Conserjeria, Mantenimiento y Mensajería S.A.(8)(9)

Guatemala

Maintenance services

100.00%

100.00%

60.00%

Mercom Bank Ltd.(8)(10)

Barbados

Banking

99.66%

99.59%

59.72%

New Alma Enterprises Ltd.(8)

Bahamas

Investments

99.66%

99.59%

59.72%

Bancolombia Puerto Rico Internacional Inc.

Puerto Rico

Banking

100.00%

100.00%

100.00%

Bancolombia Cayman S.A.(11)

Cayman Islands

Banking

100.00%

100.00%

100.00%

Banco Agrícola S.A.

El Salvador

Banking

97.36%

97.36%

97.36%

Arrendadora Financiera S.A. Arfinsa

El Salvador

Leasing

97.37%

97.37%

97.37%

Credibac S.A. de C.V.

El Salvador

Credit card services

97.36%

97.36%

97.36%

Valores Banagrícola S.A. de C.V.

El Salvador

Securities brokerage

98.89%

98.89%

98.89%

Inversiones Financieras Banco Agrícola S.A. IFBA

El Salvador

Investments

98.89%

98.89%

98.89%

Gestora de Fondos de Inversión Banagricola S.A.

El Salvador

Administers investment funds

98.89%

98.89%

98.89%

Bagrícola Costa Rica S.A.

Costa Rica

Outsourcing

99.17%

99.17%

99.17%

Bancolombia Capital Holdings USA LLC(12)

United States

Holding

100.00%

-

-

Bancolombia Capital Adviser LLC(12)

United States

Investment advisor

100.00%

-

-

Bancolombia Capital LLC(12)

United States

Securities brokerage

100.00%

-

-

(1)On November 30, 2021, Grupo Bancolombia acquired 91,791 common shares of VLIPCO S.A.S. for COP 9,474. The Grupo Bancolombia had 94.77% of the sharehold of the company. For further information see Consolidate financial statement, Note 8. Investments in associates and joint ventures and Note 9.3 Business combinations.
(2)In 2020, the Bank consolidated certain equity securities that are controlled through its subsidiary Fondo de Capital Privado Fondo Inmobiliario Colombia, due to the control definition is met according to IFRS 10. This equity securities are considered as a business, because of its capacity to generate income.
(3)Company consolidated by the Fondo de Capital Privado Fondo Inmobiliario Colombia since October 2021.
(4)The decrease in the shareholding is due to the repurchase outstanding stock carried out by the subsidiary during 2021.
(5)The trust rights were assigned in June 2021.
(6)Merger between the Banistmo S.A. (absorbing entity) and Financomer S.A. (absorbed entity) in November 2021.
(7)Investments in non-operational stage.
(8)On September 29, 2020, Grupo Bancolombia acquired 40% of the shareholdings of Grupo Agromercantil Holding (GAH), a company that owns the financial conglomerate Agromercantil of Guatemala.

(9)Companies in voluntary liquidation.
(10)On September 30, 2021, Mercom Bank Ltd shareholder authorized the beginning of an organized and gradual process to transfer of the assets and liabilities of Mercom Bank, Ltd., to Banco Agromercantil de Guatemala, S. A. or other companies of Bancolombia Group. For further information see Consolidate financial statement, Note 1. Reporting Entity.
(11)On October 5, 2020, the Board of Directors of Bancolombia Panamá (parent company of Bancolombia Cayman), authorized the decision to wind-down the business and operations of its subsidiary in Cayman. For further information see Consolidate financial statement, Note 1. Reporting entity.
(12)Companies created by Valores Bancolombia S.A. Comisionista de Bolsa in October 2021. For further information see Consolidate financial statement, Note 1. Reporting Entity.