September 9, 2024 Justin Clausen Chief Financial Officer Ames National Corporation 405 Fifth Street Ames, IA 50010 Re: Ames National Corporation Form 10-K for Fiscal Year Ended December 31, 2023 File No. 000-32637 Dear Justin Clausen: We have completed our review of your filing. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Sincerely, Division of Corporation Finance Office of Finance