SCHEDULE 13G

CUSIP No.

26745T101

1

Names of Reporting Persons

Mark A. Emalfarb

2

Check the appropriate box if a member of a Group (see instructions)

(a)

(b)

3

Sec Use Only

4

Citizenship or Place of Organization

UNITED STATES

Number of Shares Beneficially Owned by Each Reporting Person With:

5	Sole Voting Power 6,244,344.00
6	Shared Voting Power 0.00
7	Sole Dispositive Power 6,244,344.00
8	Shared Dispositive Power 0.00

9

Aggregate Amount Beneficially Owned by Each Reporting Person

6,244,344.00

10

Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)

Checkbox not checked

11

Percent of class represented by amount in row (9)

16.6 %

12

Type of Reporting Person (See Instructions)

IN

Comment for Type of Reporting Person: Includes (i) 800,000 shares of common stock underlying options present exercisable, (ii) 4,730,058 shares held through the MAE Trust U/A/D October 1, 1987 (the "MAE Trust"), and (iii) 714,286 shares underlying an 8.0% Senior Secured Convertible Promissory Note due March 8, 2027, convertible at any time at the holder's option (the "Convertible Note"), held through the MAE Trust. Mr. Emalfarb is the sole beneficiary and sole trustee of, and has sole voting and dispositive power over all shares held or otherwise owned through, the MAE Trust. The Convertible Notes are senior, secured obligations of the Issuer and its affiliates, and interest is payable quarterly in cash on the principal amount equal to 8% per annum. The Convertible Notes will mature on March 8, 2027, unless earlier converted, repurchased, or redeemed in accordance with their terms. The Convertible Notes have a conversion price of $1.40 per share of common stock. The ownership percentage is based on 36,187,798 shares of common stock of the Issuer outstanding as of November 14, 2025, as reported by the Issuer to Mr. Emalfarb.

SCHEDULE 13G

Item 1.

(a)

Name of issuer:

DYADIC INTERNATIONAL INC.

(b)

Address of issuer's principal executive offices:

1044 North U.S. Highway One, Suite 201 Jupiter Florida 33477

Item 2.

(a)

Name of person filing:

Mark A. Emalfarb

(b)

Address or principal business office or, if none, residence:

1044 North U.S. Highway One, Suite 201, Jupiter, Florida, 33447

(c)

Citizenship:

United States of America

(d)

Title of class of securities:

Common Stock

(e)

CUSIP No.:

26745T101

Item 3.

If this statement is filed pursuant to §§ 240.13d-1(b) or 240.13d-2(b) or (c), check whether the person filing is a:

(a)

Broker or dealer registered under section 15 of the Act (15 U.S.C. 78o);

(b)

Bank as defined in section 3(a)(6) of the Act (15 U.S.C. 78c);

(c)

Insurance company as defined in section 3(a)(19) of the Act (15 U.S.C. 78c);

(d)

Investment company registered under section 8 of the Investment Company Act of 1940 (15 U.S.C. 80a-8);

(e)

An investment adviser in accordance with § 240.13d-1(b)(1)(ii)(E);

(f)

An employee benefit plan or endowment fund in accordance with § 240.13d-1(b)(1)(ii)(F);

(g)

A parent holding company or control person in accordance with § 240.13d-1(b)(1)(ii)(G);

(h)

A savings associations as defined in Section 3(b) of the Federal Deposit Insurance Act (12 U.S.C. 1813);

(i)

A church plan that is excluded from the definition of an investment company under section 3(c)(14) of the Investment Company Act of 1940 (15 U.S.C. 80a-3);

(j)

A non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J). If filing as a non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J),
please specify the type of institution:

(k)

Group, in accordance with Rule 240.13d-1(b)(1)(ii)(K).

Item 4.

Ownership

(a)

Amount beneficially owned:

6,244,344

(b)

Percent of class:

16.6%

(c)

Number of shares as to which the person has:

(i) Sole power to vote or to direct the vote:

6,244,344

(ii) Shared power to vote or to direct the vote:

0

(iii) Sole power to dispose or to direct the disposition of:

6,244,344

(iv) Shared power to dispose or to direct the disposition of:

0

Item 5.

Ownership of 5 Percent or Less of a Class.

Item 6.

Ownership of more than 5 Percent on Behalf of Another Person.

Not Applicable

Item 7.

Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on by the Parent Holding Company or Control Person.

Not Applicable

Item 8.

Identification and Classification of Members of the Group.

Not Applicable

Item 9.

Notice of Dissolution of Group.

Not Applicable

Item 10.

Certifications:

Not Applicable

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

Mark A. Emalfarb

Signature:	/s/ Mark Emalfarb
Name/Title:	Self
Date:	11/14/2025