Exhibit 31.1
CERTIFICATION
I, Susan B. Washer, certify that:
| 1. | I have reviewed this Amendment No.1 to the Annual Report on Form 10-K of Applied Genetic Technologies Corporation; and | 
| 2. | Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report. | 
| Date: October 24, 2022 | By: | /s/ Susan B. Washer | ||||
| Susan B. Washer | ||||||
| Chief Executive Officer and President | ||||||
| (Principal Executive Officer) |