 
Step 1: Go to www.envisionreports.com/NAGE. Step 2: Click on Cast Your Vote or Request Materials. Step 3: Follow the instructions on the screen to log in. www.envisionreports.com/NAGE Online Go to www.envisionreports.com/NAGE or scan  the QR code — login details are located in the  shaded bar below. Shareholder Meeting Notice 0455WC Important Notice Regarding the Availability of Proxy Materials for the  Niagen Bioscience, Inc. Annual Shareholder Meeting to be Held on June 24, 2025 Under Securities and Exchange Commission rules, you are receiving this notice that the proxy materials for the annual  shareholders’ meeting are available on the Internet. Follow the instructions below to view the materials and vote online or  request a copy. The items to be voted on and location of the annual meeting are on the reverse side. Your vote is important!  This communication presents only an overview of the complete proxy materials that are available to you on the Internet. We encourage you to access and review all of the important information contained in the proxy materials before voting.  The 2025 proxy statement and annual report to shareholders are available at www.Niagenbioscience.com, Investor  Relations Section and:  Obtaining a Copy of the Proxy Materials – If you want to receive a copy of the proxy materials, you must  request one. There is no charge to you for requesting a copy. Please make your request as instructed on the  reverse side on or before June 14, 2025 to facilitate timely delivery. 2 N O T Easy Online Access — View your proxy materials and vote. When you go online, you can also help the environment by consenting to receive electronic delivery of future materials. The Sample Company Step 4: Make your selections as instructed on each screen for your delivery preferences. Step 5: Vote your shares. Votes submitted electronically must be  received by 11:59 p.m., Eastern Time on June 23, 2025. MMMMMMMMMMMM M M M M M M M M M 0 0 0 0 0 1 MR A SAMPLE DESIGNATION (IF ANY) ADD 1 ADD 2 ADD 3 ADD 4 ADD 5 ADD 6 ENDORSEMENT_LINE______________ SACKPACK_____________ 1234  5678  9012  345 C 1234567890  C O Y MMMMMMM 
 
 
 
Here’s how to order a copy of the proxy materials and select delivery preferences:  Current and future delivery requests can be submitted using the options below.  If you request an email copy, you will receive an email with a link to the current meeting materials. PLEASE NOTE: You must use the number in the shaded bar on the reverse side when requesting a copy of the proxy materials. —  Internet – Go to www.envisionreports.com/NAGE. Click Cast Your Vote or Request Materials. — Phone – Call us free of charge at 1-866-641-4276. —  Email – Send an email to investorvote@computershare.com with “Proxy Materials Niagen Bioscience, Inc.” in the subject line. Include  your full name and address, plus the number located in the shaded bar on the reverse side, and state that you want a paper copy of  the meeting materials.  To facilitate timely delivery, all requests for a paper copy of proxy materials must be received by June 14, 2025. Niagen Bioscience, Inc.’s Annual Meeting of Shareholders will be held on Tuesday, June 24, 2025 at 3:00 P.M. Pacific Time.  The meeting will be held at 10900 Wilshire Blvd. Suite 600, Los Angeles, CA 90024.  Proposals to be voted on at the meeting are listed below along with the Board of Directors’ recommendations. The Board of Directors recommend a vote FOR all the nominees listed and FOR Proposals 2, 3, 4, and 5.  1. Election of Directors:   01 - Frank Jaksch, Jr.   02 - Robert Fried   03 - Steven Rubin   04 - Wendy Yu   05 - Gary Ng   06 - Kristin Patrick   07 - Ann Cohen   08 - Hamed Shahbazi  2. Ratification of the selection of Crowe LLP as our independent registered public accounting firm for the fiscal year ending December 31, 2025  3. Approval, on an advisory basis, of the compensation of our named executive officers, as disclosed in the accompanying proxy statement  4.  Approval of amendment to the Company’s 2017 Equity Incentive Plan to increase the number of shares available for issuance by 4.75 million  shares of common stock  5.  Approval of the Company’s Employee Stock Purchase Plan   PLEASE NOTE – YOU CANNOT VOTE BY RETURNING THIS NOTICE. To vote your shares you must go online or request a paper copy of the proxy  materials to receive a proxy card. Shareholder Meeting Notice