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Exhibit 21.1

Subsidiaries of Gevo, Inc.

Name of Subsidiary

Jurisdiction

Gevo Asset, LLC

Delaware

Gevo RNG Holdco, LLC

Delaware

Gevo NW Iowa RNG, LLC

Delaware

Agri-Energy, LLC

Minnesota

Gevo Net-Zero Holdco, LLC

Delaware

Gevo Net-Zero 1, LLC

Delaware

Gevo Net-Zero Operations, LLC

Delaware

Gevo Net-Zero Asset Management, LLC

Delaware

Verity Holdings, LLC

Delaware

Cultivate Agricultural Intelligence, LLC

Florida

Gevo North Dakota Asset Management, LLC

Delaware

Gevo Intermediate HoldCo, LLC

Delaware

Net-Zero North HoldCo, LLC

Delaware

Net-Zero Richardton, LLC

Delaware

Richardton CCS, LLC

Delaware

Gevo North Dakota Operations, LLC

Delaware