Document

We consent to the incorporation by reference in this Registration Statement on Form S-3 and related prospectus of MidWestOne Financial Group, Inc. of our reports dated March 8, 2024, relating to the consolidated financial statements and the effectiveness of internal control over financial reporting of MidWestOne Financial Group, Inc., appearing in the Annual Report on Form 10-K of MidWestOne Financial Group, Inc., for the year ended December 31, 2023.

We also consent to the reference to our firm under the heading "Experts" in such Prospectus.