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Exhibit 8.1

Subsidiaries of Galapagos NV on 31/12/2024

Name of Subsidiary

    

Jurisdiction of Incorporation or Organization

Galapagos B.V.

The Netherlands

Galapagos U.K. Ltd

United Kingdom

Galapagos SASU

France

Galapagos GmbH

Switzerland

Galapagos Real Estate Belgium BV

Belgium

Galapagos Real Estate Netherlands B.V.

The Netherlands

GLPG US Holding Inc. (formerly Galapagos, Inc.)

United States

GLPG US Inc. (formerly AboundBio, Inc.)

United States

Xenometrix, Inc. (in liquidation)

United States

Galapagos Holding Pte. Ltd.

Singapore

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