March 26, 2025

Jason A. Okazaki
Chief Executive Officer and President
Assembly Biosciences, Inc.
Two Tower Place, 7th Floor
South San Francisco, California 94080

       Re: Assembly Biosciences, Inc.
           Registration Statement on Form S-3
           Filed March 20, 2025
           File No. 333-285970
Dear Jason A. Okazaki:

       This is to advise you that we have not reviewed and will not review your
registration
statement.

        Please refer to Rules 460 and 461 regarding requests for acceleration.
We remind you
that the company and its management are responsible for the accuracy and
adequacy of their
disclosures, notwithstanding any review, comments, action or absence of action
by the staff.

       Please contact Chris Edwards at 202-551-6761 with any questions.



                                                           Sincerely,

                                                           Division of
Corporation Finance
                                                           Office of Life
Sciences
cc:   P. Michelle Gasaway, Esq.