June 22, 2020
    
    
    VIA EDGAR
    
    
    U.S. Securities and Exchange Commission
    Division of Corporation Finance
    100 F Street, N.E.
    Washington, D.C. 20549
    
    
    
        
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             Attn: 
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             Ms. Laura Crotty 
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             Re: 
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             Castle Biosciences, Inc. 
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             Registration Statement on Form S-1 
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             File No. 333-239354 
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    Ladies and Gentlemen:
    
    
    In accordance with Rule 461 under the Securities Act of 1933, as amended, the undersigned registrant (the “Registrant”) hereby requests that the Securities and Exchange Commission (the “Commission”) take appropriate action to cause the above-referenced Registration Statement on
      Form S-1 (the “Registration Statement”) to become effective on June 24, 2020, at 4:30 P.M., Eastern Time, or as soon thereafter as is practicable, or at such later time as the Registrant or
      its legal counsel may orally request via telephone call to the staff. This request for acceleration is subject to your receiving a telephone call prior to such time from our legal counsel, Cooley LLP, confirming this request.
    Once the Registration Statement has been declared effective, please orally confirm that event with Phillip S. McGill of Cooley LLP at
      (858) 550‑6193 or, in his absence, Karen E. Anderson of Cooley LLP at (858) 550‑6088.
    
    
    
        
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             Very truly yours, 
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             Castle Biosciences, Inc. 
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             By: 
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             /s/ Derek J. Maetzold 
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             Derek J. Maetzold 
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             President and Chief Executive Officer 
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