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| Subsidiary | | Jurisdiction |
| ArcherDX, LLC | | Delaware |
| ArcherDX Clinical Services, Inc. | | Colorado |
| Ciitizen, LLC | | Delaware |
| Genelex India Private Limited | | India |
| Genetic Solutions LLC, d/b/a Genelex | | Pennsylvania |
| Genosity, LLC | | Delaware |
| Good Start Genetics, Inc. | | Delaware |
| Invitae Australia PTY LTD | | Australia |
| Invitae Canada Inc. | | British Colombia, Canada |
| Invitae Israel Inc Ltd. | | Israel |
| Invitae Japan, KK | | Japan |
| Invitae Latvia SIA | | Latvia |
| Invitae Medical Genetics Brasil Ltda. | | Brazil |
| Invitae Netherlands, B.V. | | Netherlands |
| Invitae (Singapore) Pte. Ltd. | | Singapore |
| Medneon LLC | | Delaware |
| Ommdom Inc. | | Delaware |
| Orbicule BVBA d/b/a Diploid | | Belgium |
| Prompt Genomics, LLC | | Delaware |
| YouScript, LLC | | Delaware |
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