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Exhibit 23.1
Consent of Independent Registered Public Accounting Firm
We hereby consent to the incorporation by reference in the Registration Statements on Form S-8 (Nos. 333-255413, 333-255440, 333-269958 and 333-277221) of DigitalOcean Holdings, Inc. of our report dated February 25, 2025 relating to the financial statements and the effectiveness of internal control over financial reporting, which appears in this Form 10-K.
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| /s/ PricewaterhouseCoopers LLP |
| Denver, Colorado |
| February 25, 2025 |