SCHEDULE 13D/A 0001104659-22-001814 0001583223 XXXXXXXX LIVE 2 Class A Common Stock, Par Value $1.00 Per Share 09/16/2025 false 0001901637 90355N101 USCB FINANCIAL HOLDINGS, INC.

2301 NW 87TH AVENUE 2301 NW 87TH AVENUE DORAL FL 33172

W. Kirk Wycoff 215-399-4650 Four Radnor Corporate Center Suite 210 Radnor PA 19087 Philip Ross Bevan, Esq. 202-295-4500 3299 K Street, N.W. Suite 100 Washington DC 20007 Kenneth B. Tabach, Esq. 2022954500 3299 K Street, N.W. Suite 100 Washington DC 20007 0001583223 N Patriot Financial Partners II, L.P. a OO N DE 0.00 1823182.00 0.00 1823182.00 1823182.00 N 10.1 PN 0001586224 N Patriot Financial Partners Parallel II, L.P. a OO N DE 0.00 212727.00 0.00 212727.00 212727.00 N 1.2 PN 0002061817 N Patriot Financial Partners GP II, L.P. a OO N DE 0.00 2035909.00 0.00 2035909.00 2035909.00 N 11.2 PN 0000949197 N WYCOFF W KIRK a OO N X1 11500.00 2035909.00 11500.00 2035909.00 2047909.00 N 11.3 IN 0001203115 N LUBERT IRA M a OO N X1 0.00 2035909.00 0.00 2035909.00 2035909.00 N 11.2 IN 0001222379 N LYNCH JAMES J a OO N X1 0.00 2035909.00 0.00 2035909.00 2035909.00 N 11.2 IN 0002061814 N Patriot Financial Partners GP II, LLC a OO N 0.00 2035909.00 0.00 2035909.00 2035909.00 N 11.2 OO 0001561111 N Deutsch James F. a OO N X1 0.00 2035909.00 0.00 2035909.00 2035909.00 N 11.2 IN Class A Common Stock, Par Value $1.00 Per Share USCB FINANCIAL HOLDINGS, INC. 2301 NW 87TH AVENUE 2301 NW 87TH AVENUE DORAL FL 33172 INTRODUCTION This Amendment No. 2 to Schedule 13D ("Amendment No. 2") is being filed to amend and supplement the statement on Schedule 13D relating to Class A Voting Common Stock, par value $1.00 per share (the "Class A Common Stock"), of USCB Financial Holdings, Inc. (the "Issuer" or the "Company") as filed with the Securities and Exchange Commission (the "SEC") on January 6, 2022 (the "Schedule 13D") and Amendment No 1 as filed with the SEC on September 4, 2025 ("Amendment No. 1"). All capitalized terms not otherwise defined herein shall have the meaning ascribed to the terms in the Initial Schedule 13D. Patriot Financial Group II beneficially owns in the aggregate 2,047,409 shares of Class A Common Stock or 11.3% of the outstanding shares. Patriot Fund II beneficially owns 1,823,182 shares of Class A Common Stock or 10.1% of the outstanding shares, with the shared power to vote and dispose the 1,823,182 shares and no sole power to vote and dispose the shares. Patriot Parallel Fund II beneficially owns 212,727 shares of Class A Common Stock or 1.2% of the outstanding shares, with the shared power to vote and dispose the 212,727 shares and no sole power to vote and dispose the shares. Each of Patriot GP II, Patriot II LLC and Messrs. Lubert, Lynch and Deutsch beneficially owns 2,035,909 shares of Class A Common Stock or 11.2% of the outstanding shares, with the shared power to vote and dispose the 2,035,909 shares and no sole power to vote and dispose the shares. W. Kirk Wycoff beneficially owns 2,047,409 shares of Class A Common Stock or 11.3% of the outstanding shares, with the shared power to vote and dispose 2,035,409 shares and the sole power to vote and dispose of 11,500 shares, which represent options granted to Mr. Wycoff as a director of the Company. Each of Patriot II GP, Patriot II LLC and Messrs. Wycoff, Lubert, Lynch and Deutsch disclaims beneficial ownership of the shares owned by the Patriot Funds, except to the extent of its or their pecuniary interest therein. On September 16, 2025, Patriot Financial Partners II, LP agreed to sell to clients of T. Rowe Price Investment Manager, Inc. (collectively, the "Purchasers") 1,119,391 shares of Class A Common Stock for $17.19 per share and Patriot Financial Partners Parallel II, LP agreed to sell to the Purchasers 130,609 shares of Class A Common Stock for $17.19 per share for an aggregate purchase price of $21,487,500. Other than the Patriot Financial Group II, no other person has the right to receive or the power to direct the receipt of dividends from, or the proceeds from the sale of, the securities of the Company referred to in this Item 5. Not applicable. The calculations for percentage of outstanding shares are based on 18,107,385 shares of Class A Common Stock outstanding as of September 15, 2025 according to information provided by the Company. Purchase Agreement dated September 16, 2025 between Patriot Financial Partners II, LP, Patriot Financial Partners Parallel II, LP and T. Rowe Price Investment Management, Inc., as investment advisor or subadvisor (exhibits excluded). Patriot Financial Partners II, L.P. /s/ W. Kirk Wycoff 09/17/2025 Patriot Financial Partners Parallel II, L.P. /s/ W. Kirk Wycoff 09/17/2025 Patriot Financial Partners GP II, L.P. /s/ W. Kirk Wycoff 09/17/2025 WYCOFF W KIRK /s/ W. Kirk Wycoff 09/17/2025 LUBERT IRA M /s/ Ira M. Lubert 09/17/2025 LYNCH JAMES J /s/ James J. Lynch 09/17/2025 Patriot Financial Partners GP II, LLC /s/ W. Kirk Wycoff 09/17/2025 Deutsch James F. /s/ James F. Deutsch 09/17/2025