Exhibit 16.1
October 21, 2025
Securities and Exchange Commission
100 F Street, N.E.
Washington, DC 20549
Commissioners:
We have read the statements made by American Strategic Investment Co., which we understand will be filed with the Securities and Exchange Commission, pursuant to Item 4.01 of Form 8-K of American Strategic Investment Co. dated October 16, 2025. We agree with the statements concerning our Firm contained therein.
Very truly yours,
| /s/ PricewaterhouseCoopers |
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PricewaterhouseCoopers LLP, PricewaterhouseCoopers Center, 300 Madison Avenue, New York, NY 10017 (646) 471 3000 |