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    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

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        <entityName>ARTELO BIOSCIENCES, INC.</entityName>
        <issuerAddress>
            <street1>505 LOMAS SANTA FE, SUITE 160</street1>
            <city>SOLANA BEACH</city>
            <stateOrCountry>CA</stateOrCountry>
            <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
            <zipCode>92075</zipCode>
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        <issuerPhoneNumber>858-925-7049</issuerPhoneNumber>
        <jurisdictionOfInc>NEVADA</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>REACTIVE MEDICAL INC.</previousName>
            <previousName>KNIGHT KNOX DEVELOPMENT CORP.</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

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            <relatedPersonName>
                <firstName>Gregory</firstName>
                <middleName>D.</middleName>
                <lastName>Gorgas</lastName>
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            <relatedPersonAddress>
                <street1>c/o Artelo Biosciences, Inc.</street1>
                <street2>505 Lomas Santa Fe, Suite 160</street2>
                <city>Solana Beach</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>92075</zipCode>
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                <relationship>Director</relationship>
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                <firstName>Connie</firstName>
                <lastName>Matsui</lastName>
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                <street2>505 Lomas Santa Fe, Suite 160</street2>
                <city>Solana Beach</city>
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                <zipCode>92075</zipCode>
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                <street2>505 Lomas Santa Fe, Suite 160</street2>
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                <lastName>Emanuele</lastName>
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                <firstName>Tamara</firstName>
                <middleName>A.</middleName>
                <lastName>Favorito</lastName>
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        <relatedPersonInfo>
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                <firstName>Mark</firstName>
                <lastName>Spring</lastName>
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    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2026-03-12</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isDebtType>true</isDebtType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>113000</totalOfferingAmount>
            <totalAmountSold>113000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>1</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
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                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
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            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
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            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>ARTELO BIOSCIENCES, INC.</issuerName>
                <signatureName>Gregory D. Gorgas</signatureName>
                <nameOfSigner>Gregory D. Gorgas</nameOfSigner>
                <signatureTitle>President &amp; CEO</signatureTitle>
                <signatureDate>2026-03-27</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>