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S-8 S-8 EX-FILING FEES 0001623526 Stoke Therapeutics, Inc. N/A Fees to be Paid Fees to be Paid 0001623526 2026-03-12 2026-03-12 0001623526 1 2026-03-12 2026-03-12 0001623526 2 2026-03-12 2026-03-12 iso4217:USD xbrli:pure xbrli:shares

Calculation of Filing Fee Tables

S-8

Stoke Therapeutics, Inc.

Table 1: Newly Registered Securities

Security Type

Security Class Title

Fee Calculation Rule

Amount Registered

Proposed Maximum Offering Price Per Unit

Maximum Aggregate Offering Price

Fee Rate

Amount of Registration Fee

1 Equity Common stock, par value $0.0001 per share Other 2,356,879 $ 38.61 $ 90,999,098.19 0.0001381 $ 12,566.98
2 Equity Common stock, par value $0.0001 per share Other 589,219 $ 32.82 $ 19,338,167.58 0.0001381 $ 2,670.60

Total Offering Amounts:

$ 110,337,265.77

$ 15,237.58

Total Fee Offsets:

$ 0.00

Net Fee Due:

$ 15,237.58

Offering Note

1

(1) Represents additional shares of Common Stock to be registered and available for grant under the Registrant's 2019 Equity Incentive Plan (the "2019 Plan") resulting from the annual automatic increase in shares available for issuance under the 2019 Plan pursuant to the provision of the 2019 Plan providing for an annual automatic increase in the number of shares reserved for issuance under the 2019 Plan. (2) Estimated pursuant to Rules 457(c) and 457(h) under the Securities Act, solely for the purposes of calculating the registration fee and based on the average of the high and low prices of Common Stock as reported on The Nasdaq Stock Market LLC ("Nasdaq") on March 11, 2026, which date is within five business days prior to the filing of this Registration Statement.

2

(1) Represents additional shares of Common Stock to be registered and available for grant under the Registrant's 2019 Employee Stock Purchase Plan (the "Purchase Plan") resulting from the annual automatic increase in shares available for issuance under the Purchase Plan pursuant to the provision of the Purchase Plan providing for an annual automatic increase in the number of shares reserved for issuance under the Purchase Plan. (2) Estimated pursuant to Rules 457(c) and 457(h) under the Securities Act, solely for the purposes of calculating the registration fee and based on the average of the high and low prices of Common Stock as reported on Nasdaq on March 11, 2026, which date is within five business days prior to the filing of this Registration Statement, multiplied by 85%, which is the percentage of the price per share applicable to purchases under the Purchase Plan.
Table 2: Fee Offset Claims and Sources ☑Not Applicable
Registrant or Filer Name Form or Filing Type File Number Initial Filing Date Filing Date Fee Offset Claimed Security Type Associated with Fee Offset Claimed Security Title Associated with Fee Offset Claimed Unsold Securities Associated with Fee Offset Claimed Unsold Aggregate Offering Amount Associated with Fee Offset Claimed Fee Paid with Fee Offset Source
Rule 457(p)
Fee Offset Claims N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Fee Offset Sources N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A