Exhibit 32.1
CERTIFICATION PURSUANT TO
18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO
SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002
In connection with the Annual Report of Metacrine, Inc. (the “Company”) on Form 10-K for the fiscal year ended December 31, 2021, as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I certify pursuant to 18 U.S.C. §1350, as adopted pursuant to §906 of the Sarbanes-Oxley Act of 2002, that to the best of my knowledge:
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 | (1) | the Report fully complies with the requirements of section 13(a) or 15(d) of the Securities Exchange Act of 1934, and | 
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 | (2) | the information contained in the Report fairly presents, in all material respects, the financial condition and result of operations of the Company. | 
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| Date: March 30, 2022 | 
 | By: | /s/ Preston Klassen, M.D. | 
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 | Preston Klassen, M.D. | 
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 | President and Chief Executive Officer | 
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 | (Principal Executive Officer) | 
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| Date: March 30, 2022 | 
 | By: | /s/ Patricia Millican | 
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 | Patricia Millican | 
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 | Chief Financial Officer | 
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 | (Principal Financial and Accounting Officer) | 
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