| Name of Subsidiary | State or Country of Incorporation or Organization | |||||||
| IsoTis International SARL | Switzerland | |||||||
| IsoTis OrthoBiologics, Inc. | Washington | |||||||
| IosTis, Inc. | Delaware | |||||||
| SeaSpine, Inc. | Delaware | |||||||
| SeaSpine Sales LLC | Delaware | |||||||
| SeaSpine Orthopedics Corporation | Delaware | |||||||
| Theken Spine, LLC | Ohio | |||||||
| SeaSpine Orthopedics IntermediateCo, Inc. | Delaware | |||||||
| Project Maple Leaf Holdings ULC | British Columbia | |||||||
| 7D Surgical ULC | British Columbia | |||||||
| 7D Surgical USA Inc. | Delaware | |||||||
| 7D Surgical International Inc. | Barbados | |||||||