Exhibit 8.1
Pyxis Tankers Inc.
List of Subsidiaries*
| Company Name | Jurisdiction of Incorporation |
| Secondone Corporation Ltd. | Malta |
| Thirdone Corporation Ltd. | Malta |
| Fourthone Corporation Ltd. | Malta |
Sixthone Corp.** Seventhone Corp. |
Republic of the Marshall Islands Republic of the Marshall Islands |
| Eighthone Corp. | Republic of the Marshall Islands |
| Maritime Technologies Corp. | Delaware, U.S.A. |
*Each subsidiary is 100% owned by Pyxis Tankers Inc.
** Pyxis Delta was sold to an unaffiliated third party on January 13, 2020.