Exhibit 8.1
Pyxis Tankers Inc.
List of Subsidiaries*
| Company Name | Jurisdiction of Incorporation | |
| Secondone Corporation Ltd. ** | Republic of the Marshall Islands | |
| Thirdone Corporation Ltd. ** | Republic of the Marshall Islands | |
| Fourthone Corporation Ltd. ** | Malta | |
| Sixthone Corp. ** | Republic of the Marshall Islands | |
| Seventhone Corp. | Republic of the Marshall Islands | |
| Eighthone Corp. | Republic of the Marshall Islands | |
| Tenthone | Republic of the Marshall Islands | |
| Eleventhone | Republic of the Marshall Islands | |
| Maritime Technologies Corp. | Delaware, U.S.A. |
*Each subsidiary is 100% owned by Pyxis Tankers Inc.
** “Pyxis Delta”, “Northsea Alpha”, “Northsea Beta” and “Pyxis Malou” were sold to unaffiliated third parties on January 13, 2020, January 28, 2022, March 1, 2022, and March 23, 2023 respectively.