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Exhibit 21.1

Subsidiaries of Rhythm Pharmaceuticals, Inc.

Entity
Jurisdiction of Organization or Incorporation
Rhythm Securities Corp.
Massachusetts
Rhythm Pharmaceuticals Canada Inc.
Canada
Rhythm Pharmaceuticals France SAS
France
Rhythm Pharmaceuticals Germany GmbH
Germany
Rhythm Pharmaceuticals Italy S.r.l.
Italy
Rhythm Pharma K.K.
Japan
Rhythm Pharmaceuticals Spain, SL
Spain
Rhythm Pharmaceuticals Switzerland GmbH
Switzerland
Rhythm Pharmaceuticals Netherlands B.V.
The Netherlands
Xinvento B.V.
The Netherlands
Rhythm Pharmaceuticals UK Limited
United Kingdom