Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in this Registration Statement on Form S-8 of our report dated March 23, 2022, relating to the financial statements of Aptinyx Inc. appearing in the Annual Report on Form 10-K of Aptinyx Inc. for the year ended December 31, 2021.

/s/ Deloitte & Touche LLP

Chicago, Illinois 

March 23, 2022