SCHEDULE 13G/A 0001493152-25-006352 0001698148 XXXXXXXX LIVE 2 Common stock, par value $0.0001 per share 06/30/2025 0001831868 SeaStar Medical Holding Corp 81256L203 3513 Brighton Blvd Suite 410 Denver CO 80216 Rule 13d-1(b) Ayrton Capital LLC b DE 294361.00 0.00 294361.00 0.00 294361.00 N 2.6 IA Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B b E9 294361.00 0.00 294361.00 0.00 294361.00 N 2.6 CO Waqas Khatri b X1 294361.00 0.00 294361.00 0.00 294361.00 N 2.6 IN SeaStar Medical Holding Corp 3513 Brighton Blvd, Suite 410, Denver, COLORADO, 80216. (i) Ayrton Capital LLC; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B; and (iii) Waqas Khatri (i) Ayrton Capital LLC, 55 Post Rd West, 2nd Floor Westport, CT 06880; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B, Suite #7 Grand Pavilion Commercial Centre, 802 West Bay Road, Grand Cayman, P.O. Box 10250, Cayman Islands; and (iii) Waqas Khatri 55 Post Rd West, 2nd Floor Westport, CT 06880 (i) Ayrton Capital LLC: United States; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B: Cayman Islands; and (iii) Waqas Khatri: United States N IA Ayrton Capital LLC: 294,361; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B: 296,361; and (iii) Waqas Khatri: 294,361. Represents 294,361 shares of Common Stock issuable on the exercise of certain warrants (the "Warrants") held by the Reporting Persons. The issuable shares of Common Stock related to the exercise of the Warrants are subject to a 9.99% beneficial ownership blocker. The shares reported herein represent Common Stock of SeaStar Medical Holding Corporation (the "Issuer") held by Alto Opportunity Master Fund, SPC- Segregated Master Portfolio B, a Cayman Islands exempted company (the "Fund"). The Fund is a private investment vehicle for which Ayrton Capital LLC, a Delaware limited liability company (the "Investment Manager"), serves as the investment manager. Waqas Khatri serves as the managing member of the Investment Manager (all of the foregoing, collectively, the "Reporting Persons"). The percentages below are based on (i) 11,203,752 shares of Common Stock of the Issuer that were outstanding as of June 12, 2025; and (ii) 294,361 shares of Common Stock issuable on the exercise of the Warrants held by the Reporting Persons. The amount of shares outstanding was based upon a statement in the Issuer's Form 424B4 Prospectus filing on June 23, 2025. For the sake of clarity, the holdings of the Reporting Persons reported herein are as of June 30, 2025. (i) Ayrton Capital LLC: 2.56%; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B: 2.56%; and (iii) Waqas Khatri: 2.56%. (i) Ayrton Capital LLC: 294,361; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B: 294,361; and (iii) Waqas Khatri: 294,361 (i) Ayrton Capital LLC: 0; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B: 0; and (iii) Waqas Khatri: 0 (i) Ayrton Capital LLC: 294,361; Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B: 294,361; Waqas Khatri: 294,361 (i) Ayrton Capital LLC: 0; (ii) Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B: 0; (iii) Waqas Khatri: 0 Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under § 240.14a-11. Ayrton Capital LLC /s/ Waqas Khatri 08/13/2025 Alto Opportunity Master Fund, SPC - Segregated Master Portfolio B /s/ Waqas Khatri 08/13/2025 Waqas Khatri /s/ Waqas Khatri 08/13/2025