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Exhibit 31.4

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CERTIFICATIONS UNDER SECTION 302

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I, Johnny Ek, certify that:

1.I have reviewed this Amendment No. 1 to the Annual Report on Form 10-K of Akoya Biosciences, Inc.;

2.Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report;

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Date: April 28, 2025

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/s/ Johnny Ek

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Johnny Ek

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Chief Financial Officer

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(principal financial officer and principal accounting officer)

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