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SCHEDULE 13D/A 0001938373 XXXXXXXX LIVE 1 Class B Common Stock, par value $0.01 per share 06/05/2025 false 0001713863 75062E106 RAFAEL HOLDINGS, INC. 520 Broad Street Newark NJ 07102 Shmuel Jonas (973) 438-4800 I9 Plus, LLC 153 Booth Avenue Englewood NJ 07631 0001938373 N I9 Plus, LLC b WC N X1 3616431.00 0.00 3616431.00 0.00 3616431.00 N 7 OO Class B Common Stock, par value $0.01 per share RAFAEL HOLDINGS, INC. 520 Broad Street Newark NJ 07102 This Amendment No. 1 to Schedule 13D (this "Amendment") supplements and amends the information contained in the Schedule 13D originally filed with the Securities and Exchange Commission (the "Commission") on July 18, 2025, (the "Schedule 13D"). Terms used but not defined herein shall have the meanings ascribed thereto in the Schedule 13D. This Amendment No. 1 amends Items 3, 4 and 5, as set forth below. The matters set forth in Item 4 of this Amendment are incorporated in this Item 3 by reference as if fully set forth herein. Item 4 is hereby amended and supplemented as follows: On August 5, 2022, I9 Plus sold 33,100 shares of Class B Common Stock, pursuant to a Rule 10b5-1 plan adopted on July 19, 2022 (the "10b5-1 Plan"). On August 8, 2022, I9 Plus sold 14,787 shares of Class B Common Stock, pursuant to the 10b5-1 Plan. On August 9, 2022, I9 Plus sold 64,674 shares of Class B Common Stock, pursuant to the 10b5-1 Plan. On June 5, 2025, pursuant to a Standby Purchase Agreement, entered into on May 6, 2025, between Rafael Holdings, Inc. (the "Company") and Howard S. Jonas ("H. Jonas"), in connection with a rights offering undertaken by the Company, I9 Plus purchased 390,625 shares of Class B Common Stock. Items 5 is hereby amended by deleting it in its entirety and substituting the following therefor: As of the date hereof, I9 Plus is the beneficial owner, and has the sole power to vote, or to direct the voting, and to dispose, or direct the disposition, of 3,616,431 shares of the Company's Class B Common stock. This number represents approximately 7% of the issued and outstanding Shares as of June 9, 2025 (including shares issued pursuant to the Standby Purchase Agreement). I9 Plus, LLC /s/ Shmuel Jonas Shmuel Jonas, Manager 06/09/2025