Exhibit 21.1
LIST OF SUBSIDIARIES
| Company Name | Jurisdiction of Incorporation | |
| Save Foods Ltd.(1) | Israel | |
| NTWO OFF Ltd. (2) | Israel | |
| NITO Renewable Energy, Inc. (3) | Nevada |
| (1) | Save Foods Ltd. is the 98.48% owned subsidiary of N2OFF, Inc. | |
| (2) | NTWO OFF Ltd. is the 60% owned subsidiary of N2OFF, Inc. | |
| (3) | NITO Renewable Energy, Inc. is the 100% owned subsidiary of N2OFF, Inc., and which owns 70% of SB Impact 4 Ltd., a company organized under the laws of Italy |