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Exhibit 21.1

Subsidiaries of DocGo Inc.

 The registrant’s subsidiaries and affiliates as of December 31, 2025 are included in the list below.

Name						Jurisdiction
AF DE LNZ LLC						Delaware, United States
AF WI LNZ, LLC						Wisconsin, United States
AF WI, LLC						Wisconsin, United States
Ambulnz AL, LLC						Alabama, United States
Ambulnz CO, LLC						Colorado, United States
Ambulnz Community Partners Ltd						England and Wales
Ambulnz GA, LLC						Georgia, United States
Ambulnz Holdings, LLC						Delaware, United States
Ambulnz NJ, LLC						New Jersey, United States
Ambulnz NJ-PA, LLC						Delaware, United States
Ambulnz NY 2, LLC						Delaware, United States
Ambulnz NY 3, LLC						Delaware, United States
Ambulnz NY 5, LLC						New York, United States
Ambulnz NY 6, LLC						New York, United States
Ambulnz NY, LLC						Delaware, United States
Ambulnz PA, LLC						Pennsylvania, United States
Ambulnz PA2, LLC						Pennsylvania, United States
Ambulnz RE 1, LLC						New York, United States
Ambulnz TN, LLC						Tennessee, United States
Ambulnz TX, LLC						Texas, United States
Ambulnz UK LTD						England and Wales
Ambulnz UK, LLC						Delaware, United States
Ambulnz WI, LLC						Wisconsin, United States
Ambulnz, Inc.						Delaware, United States
Ambulnz Institute for Pre-Hospital Medicine LLC						Pennsylvania, United States
Ambulnz-FMC North America LLC						Delaware, United States
Ambulnz-PA WC, LLC						Pennsylvania, United States
ARM Insurance, Inc.						Vermont, United States
AZ Ambulette, LLC						New York, United States
Cardiac RMS LLC						New York, United States
Century Ambulance Services Inc.						New York, United States
Clarion Medical, LLC						Pennsylvania, United States
Community Ambulance Service Ltd						England and Wales
CRMS DG, LLC						New York, United States
Dara Technologies, LLC						Delaware, United States
DocGo EMS Training Center, LLC						New York, United States
DocGo Mobile Health Supplies, LLC						New York, United States
EMS DIRECT, LLC						Texas, United States
Exceptional Medical Transportation, LLC						New Jersey, United States
Government Medical Services, LLC						Delaware, United States
Heartwatch Solutions, LLC						California, United States
Healthworx LLC						Delaware, United States
LabGo Holdings, LLC						Delaware, United States

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LJH Ambulance, Inc.						Wisconsin, United States
Location Medical Services Limited						England and Wales
National Providers Association, LLC						Delaware, United States
On Duty Staffing Group, LLC						Delaware, United States
Professional Technicians, LLC						Delaware, United States
Rapid Temps, LLC						New Mexico, United States
Rapid Reliable Care CA by DocGo, LLC						California, United States
Rapid Reliable Care FL by DocGo, LL						Florida, United States
Rapid Reliable Care NY by DocGo, LLC						New York, United States
Rapid Reliable Care by DocGo, LLC						Delaware, United States
Rapid Reliable Care CA Transport, LLC						California, United States
Ryan Bros. Ambulance, LLC						Wisconsin, United States
Ryan Bros. Fort Atkinson, LLC						Wisconsin, United States
SteadyMD, LLC						Delaware, United States
Virtual Care Management, LLC						Delaware, United States