<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001825452</cik>
        <entityName>Onfolio Holdings, Inc</entityName>
        <issuerAddress>
            <street1>1007 NORTH ORANGE STREET</street1>
            <street2>4TH FLOOR</street2>
            <city>WILMINGTON</city>
            <stateOrCountry>DE</stateOrCountry>
            <stateOrCountryDescription>DELAWARE</stateOrCountryDescription>
            <zipCode>19801</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>(682) 990-6920</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>Onfolio Holdings Inc</previousName>
            <previousName>Onfolio Holdings, Inc.</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Dominic</firstName>
                <lastName>Wells</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1007 NORTH ORANGE STREET 4TH FLOOR</street1>
                <city>WILMINGTON</city>
                <stateOrCountry>DE</stateOrCountry>
                <stateOrCountryDescription>DELAWARE</stateOrCountryDescription>
                <zipCode>19801</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
                <relationship>Executive Officer</relationship>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Adam</firstName>
                <lastName>Trainor</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1007 NORTH ORANGE STREET 4TH FLOOR</street1>
                <city>WILMINGTON</city>
                <stateOrCountry>DE</stateOrCountry>
                <stateOrCountryDescription>DELAWARE</stateOrCountryDescription>
                <zipCode>19801</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Andrew</firstName>
                <lastName>Lawrence</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1007 NORTH ORANGE STREET 4TH FLOOR</street1>
                <city>WILMINGTON</city>
                <stateOrCountry>DE</stateOrCountry>
                <stateOrCountryDescription>DELAWARE</stateOrCountryDescription>
                <zipCode>19801</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>David</firstName>
                <lastName>McKeegan</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1007 NORTH ORANGE STREET 4TH FLOOR</street1>
                <city>WILMINGTON</city>
                <stateOrCountry>DE</stateOrCountry>
                <stateOrCountryDescription>DELAWARE</stateOrCountryDescription>
                <zipCode>19801</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Robert</firstName>
                <lastName>Lipstein</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1007 NORTH ORANGE STREET 4TH FLOOR</street1>
                <city>WILMINGTON</city>
                <stateOrCountry>DE</stateOrCountry>
                <stateOrCountryDescription>DELAWARE</stateOrCountryDescription>
                <zipCode>19801</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Mark</firstName>
                <middleName>N.</middleName>
                <lastName>Schwartz</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1007 NORTH ORANGE STREET 4TH FLOOR</street1>
                <city>WILMINGTON</city>
                <stateOrCountry>DE</stateOrCountry>
                <stateOrCountryDescription>DELAWARE</stateOrCountryDescription>
                <zipCode>19801</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other Technology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>$5,000,001 - $25,000,000</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
            <item>4a5</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2025-10-21</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>50000</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>1000000</totalOfferingAmount>
            <totalAmountSold>1000000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>11</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
        </useOfProceeds>
        <signatureBlock>
            <signature>
                <issuerName>Onfolio Holdings, Inc</issuerName>
                <signatureName>/s/Dominic Wells</signatureName>
                <nameOfSigner>Dominic Wells</nameOfSigner>
                <signatureTitle>CEO</signatureTitle>
                <signatureDate>2025-10-24</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>