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Consent of Independent Registered Public Accounting Firm
We consent to the incorporation by reference in the following Registration Statements:
(1)Registration Statement (Form S-3 No. 333-269569) of IO Biotech, Inc.,
(2)Registration Statement (Form S-8 No. 333-262587) pertaining to the 2021 Equity and Incentive Plan and 2021 Employee Stock Purchase Plan of IO Biotech, Inc.,
(3)Registration Statement (Form S-8 No. 333-269597) pertaining to the IO Biotech, Inc. 2021 Equity and Incentive Plan,
(4)Registration statement (Form S-3 No. 333-274267) of IO Biotech, Inc., and
(5)Registration Statement (Form S-8 No. 333-277826) pertaining to the IO Biotech, Inc. 2021 Equity and Incentive Plan, the IO Biotech, Inc. 2023 Inducement Award Plan, and the Option Inducement Award;
of our report dated March 31, 2025, with respect to the consolidated financial statements of IO Biotech, Inc. included in this Annual Report (Form 10-K) of IO Biotech, Inc. for the year ended December 31, 2024.
/s/ EY Godkendt Revisionspartnerselskab
Copenhagen, Denmark
March 31, 2025